Fig. 6

Composition of rigid polysaccharide probed by high-resolution ssNMR. (A) Overlay CP spectra of positive control and ∆mpkA mutant, with the rigid polysaccharide shown in orange and black spectra. The glucan types and its carbon numbers are abbreviated, and color coded as: α-1,3glucan (A, green), (β-1,3-glucan (B, blue), chitin (Ch, orange), chitosan (Cs, pink). (B) The 2D ¹³C-¹³C CORD, correlation explores the spatial arrangement of cross-linkages among α-1,3-glucan, β-1,3-glucan, chitin, and chitosan. (C) The molar composition of these rigid components is determined by analyzing the peak volumes in the 2D ¹³C-¹³C CORD spectra