Fig. 7
Composition of mobile carbohydrates. (A) Overlay of 1D spectra for the control (A1405) and ΔmpkA mutant (A1404), shown in orange and black, representing the highly mobile region, semi-dynamic region, and cumulative region using INEPT, 2s DP, and 35s DP experiments. The glucan types, their monomers, and carbon numbers are labeled and color-coded as follows: galactofuranose (Galf, pink), α-1,2-mannose (Mn1,2, light brown), α-1,6-mannose (Mn1,6, brown), galactopyranose (Galp, yellow), and N-acetyl galactosamine (GalNAc, magenta). (B) The through-bond correlations 2D 1 13C-13C -DP J INADEQUATE correlation spectrum, shown in orange and grey for positive control and mutant. (C) The molar composition of these mobile components determined by analyzing the peak volumes in the 2D DP-INADEQUATE spectra. The GM molecules are color-coded in pink shades, while the GAG components are shaded in orange in the donut graph. The legend corresponds to the abbreviations, linking the monomers to their assignments in the INADEQUATE spectra