Fig. 1
From: Quantification of fungal biomass in mycelium composites made from diverse biogenic side streams
Scheme of the mycelium quantification methodology. Steps include DNA extraction from fungal mycelium grown on beech wafers (brown pathway) and PDA (blue pathway) and measurement of the concentration with a fluorometer. The amount of DNA contained per mass of mycelium from wood wafers was defined as conversion factor ξ. DNA extracted from mycelium grown on PDA was used to create a standard curve relating DNA concentration to Cq values. The green pathway shows the composite fabrication, cutting of a representative cylinder for DNA extraction from the center, and qPCR of diluted template samples to quantify fungal DNA based on the standard curve. Finally, the detected amount of fungal DNA was translated to fungal biomass via the conversion factor ξ