Table 2 Selection conditions applied in SELEX-2
Whole-cell SELEX-2 | ||||||||
|---|---|---|---|---|---|---|---|---|
Round | ssDNA | Positive selection | Reaction volume (µL) | Competitors | Washes | Counter-selection | ||
pmol | Final conc (nM) | Target strains | Number of conidia | |||||
1 | 1800 | 7200 | ATCC 1015, CBS 554.65 | 107 | 250 | – | 1 | – |
2 | 25 | 100 | ATCC 1015, CBS 554.65 | 107 | 250 | – | 1 | – |
3 | 25 | 100 | ATCC 1015, CBS 554.65 | 107 | 250 | BSA, 4sDNA | 2 | – |
4 | 10 | 100 | ATCC 1015, CBS 554.65 | 107 | 100 | – | 2 | A. tubingensis MA 3973 |
5 | 8 | 100 | ATCC 1015, CBS 554.65 | 107 | 80 | BSA, 4sDNA | 3 | A. tubingensis MA 3973 |
6 | 8 | 100 | ATCC 1015, CBS 554.65 | 107 | 80 | BSA, 4sDNA | 3 | A. tubingensis MA 3973 |
7 | 8 | 100 | ATCC 1015, CBS 554.65 | 107 | 80 | BSA, 4sDNA | 3 | A. tubingensis MA 3973 |
8 | 8 | 80 | ATCC 1015, CBS 554.65 | 107 | 100 | BSA, 4sDNA | 6 | A. tubingensis MA 3973 |
9-N | 8 | 80 | ATCC 1015, CBS 554.65 | 107 | 100 | BSA, 4sDNA | 6 | – |
9-T | 8 | 80 | A. tubingensis MA 3973 | 107 | 100 | BSA, 4sDNA | 6 | – |