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Fig. 8 | Fungal Biology and Biotechnology

Fig. 8

From: Development of a whole-cell SELEX process to select species-specific aptamers against Aspergillus niger

Fig. 8

Specificity evaluation of selected aptamers by means of qPCR (A) or fluorescence (B) measurements. A: recovered ssDNA after binding assays with A. niger, A. tubingensis and A. nidulans conidia was quantified by qPCR and is shown in molecules/µL. Samples were measured in technical duplicates. The mean value between biological and technical replicates is displayed after subtracting the mean value of the blank samples (only aptamers, no conidia). The number of biological and technical replicates performed is indicated in white in each bar. Error bars represent max. and min. values. The predicted secondary structure of each aptamer was obtained with RNAFold and is shown on top of the figure. B: confirmation of the species-specificity of three selected aptamers by fluorescent measurements upon elution. Fluorescence of the recovered and eluted ssDNA after binding assays with A. niger, A. tubingensis and A. nidulans conidia was measured at a Tecan Spark reader. Samples were measured in duplicates. The mean value between biological and technical replicates is displayed after subtracting the mean value of the blank samples (only conidia, no aptamer). Error bars represent max. and min. values. Asterisks indicate aptamers showing significantly different binding (p-value < 0.05) between different strains

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Fungal Biology and Biotechnology

ISSN: 2054-3085

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