Fig. 4

Detection of SLAC variants from the culture medium of A. niger transformants. (A) SDS-PAGE of culture medium of selected co-transformants stained with Sypro Ruby staining solution. (B) Zymogram analysis of culture medium from selected co-transformants showing activity of SLAC variants on the gel overlayed with DMPPDA substrate. (C) Western blot analysis of culture medium from selected co-transformants using SLAC-antibody. 1: protein marker, 2: CBM-SLAC, 3: SLAC, 4: A. niger MGG029 with empty vector (negative control). The same amount of protein samples was loaded on the gel