Fig. 2
From: Genomic deletions in Aureobasidium pullulans by an AMA1 plasmid for gRNA and CRISPR/Cas9 expression
Sequencing results of obtained transformants. A schematic drawing of the URA3 locus is given with the target of the CRISPR/Cas9 system. Red line and letters, PAM sequence; green line and letters, sequence complementary to the gRNA; purple letters, nucleotides being part of the URA3 locus; blue letters, nucleotides upstream and downstream of the URA3 locus; pink, italic letters, inserted nucleotides; Strain, strains analyzed; WT, wild-type; strain numbers starting with 1, transformants obtained from the first transformation; strain numbers starting with 2, transformants obtained from the second transformation; Start, genomic position in the WT. Sequence, obtained sequencing result; End, genomic position in the WT; Del, number of nucleotides deleted