Fig. 5

HPLC analysis of rasfonin between a 25 µg/mL rasfonin standard (green, A), the wildtype isolate NG_p51 (blue, B left) and the CgPKS4 knock-out strain NG_p51∆CgPKS4 (orange, C left). All 3 knock-out strains and all replicates showed the same absence of rasfonin, but only one is shown here. Spiked samples are shown on the right panel and are indicated by a green peak (NG_p51, B right; NG_p51∆CgPKS4, C right). The retention times in minutes are shown next to the peaks