Fig. 6
From: An overview on current molecular tools for heterologous gene expression in Trichoderma
CRISPR/Cas9-mediated genome editing. The Cas9 endonuclease is programmed by the guide RNA (gRNA) to introduce a double strand (DSB) in a 20Â bp long target site, directly adjacent to a protospacer adjacent motif (PAM). The DSB can be repaired by the error-prone non-homologous end joining (NHEJ) or via homologous direct repair (HDR), if a suitable donor DNA is present