Table 2 Primers used in this study
From: A new vector for efficient gene targeting to the pyrG locus in Aspergillus niger
Primer name | Sequence 5’-3’ | Used for |
|---|---|---|
ABpyrGP12for-EcoRI-AscI | CGGAATTCGG CGCGCCCGGC TGACGTTACC ACCACT* | pyrG-3’ UTR PCR (Figure 2A) |
ABpyrGP10rev-NotI | AAGGAAAAAA GCGGCCGCAG TCAGACCTAA TGCCTCGGG | pyrG-3’ UTR PCR (Figure 2A) |
ABpyrGP11for-NotI | AAGGAAAAAA GCGGCCGCCG TCGCGTGATA AGGGTTG | 3’ pyrG PCR (Figure 2A) |
ABpyrGP13rev-SacI-AscI | CGAGCTCGGC GCGCCTCGGG TCAATTTCCT CTGTTG | 3’ pyrG PCR (Figure 2a) |
SL1 | ATTTGCGGCC GCAGAACGCC GAGAAGAACT GG | PgpdA-mluc PCR (Figure 2A) |
TtrpCP2rev-NotI | AAGGAAAAAA GCGGCCGCTC TAGAAAGAAG GATTACCTC | PgpdA-mluc PCR (Figure 2A) |